This study aims to analyze the examination trend of questions related to the field of biology education among the questions of the secondary biology teacher appointment examination from 2014 to 2024. The analysis of examination question trends was conducted by using the contents of the evaluation area and the sub-evaluation content elements of the biology subject suggested by the Korea Institute for Curriculum Evaluation (KICE) as a framework. The results of the study are as follows. First, only 75% of the evaluation content elements were linked to the set questions, and it was found that only biology teaching-learning theory, biology teaching-learning practice, and biology teaching-learning evaluation areas had questions linked to all evaluation content elements. Second, the biology teachinglearning theory, biology teaching-learning practice, and biology inquiry areas accounted for 67% of the total questions. Third, it was found that no question was set in the biology education environment and support area. These results show that the secondary biology teacher appointment exam is biased towards certain areas and evaluation content elements. Furthermore, since the evaluation area and evaluation content elements also affect the education for the professionalism of prospective biology teachers, it shows the need to comprehensively review and improve the content of the evaluation area and evaluation content elements, considering teachers’ competency and educational factors for the successful progress of field education.