The purpose of this study is to compare and analyze the brain connectivity in virtual reality (VR) and book-type learning in life science. Twenty-four types of life science VR contents were composed and the same contents were devised as a printed matter. The results of analyzing the functional connectivity of the brain in VR based learning higher than book based learning are as follows. The motivation factors have increased left and right hemispheres of the orbital prefrontal cortex, inferior frontal cortex, and putamen. The immersion factors have increased the functional connectivity of the orbital prefrontal cortex and inferior frontal gyrus The social factors have increased the functional connectivity of temporal pole, inferior parietal lobule and angular. The addiction factors have increased the functional connectivity of amygdala and putamen. The dizziness factors have increased the functional connectivity of inferior temporal gyrus and rolandic operculum. Based on the results of the above research, when conducting classes with VR, utilizing differences in brain functional connectivity between VR and book-type learning, the direction of class application can be presented that is specific for teaching-learning purposes and effectively reflects the characteristics of VR and book-type learning.